KMID : 0191120130280071071
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Journal of Korean Medical Science 2013 Volume.28 No. 7 p.1071 ~ p.1076
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Quantitative in Vivo Detection of Brain Cell Death after Hypoxia Ischemia Using the Lipid Peak at 1.3 ppm of Proton Magnetic Resonance Spectroscopy in Neonatal Rats
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Ahn So-Yoon
Yoo Hye-Soo Lee Jang-Hoon Sung Dong-Kyung Jung Yu-Jin Sung Se-In Lim Keun-Ho Chang Yun-Sil Lee Jung-Hee Kim Ki-Soo Park Won-Soon
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Abstract
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This study was performed to determine the accuracy of proton magnetic spectroscopy (1H-MRS) lipid peak as a noninvasive tool for quantitative in vivo detection of brain cell death. Seven day-old Sprague Dawley rats were subjected to 8% oxygen following a unilateral carotid artery ligation. For treatment, cycloheximide was given immediately after hypoxic ischemia (HI). Lipid peak was measured using 1H-MRS at 24 hr after HI, and then brains were harvested for fluorocytometric analyses with annexin V/propidium iodide (PI) and fluorescent probe JC-1, and for adenosine-5'-triphosphate (ATP) and lactate. Increased lipid peak at 1.3 ppm measured with 1H-MRS, apoptotic and necrotic cells, and loss of mitochondrial membrane potential (¥Ä¥×) at 24 hr after HI were significantly improved with cycloheximide treatment. Significantly reduced brain ATP and increased lactate levels observed at 24 hr after HI showed a tendency to improve without statistical significance with cycloheximide treatment. Lipid peak at 1.3 ppm showed significant positive correlation with both apoptotic and necrotic cells and loss of ¥Ä¥×, and negative correlation with normal live cells. Lipid peak at 1.3 ppm measured by 1H-MRS might be a sensitive and reliable diagnostic tool for quantitative in vivo detection of brain cell death after HI.
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KEYWORD
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Hypoxia-Ischemia, Brain, Animals, Newborn, Magnetic Resonance Spectroscopy, Flow Cytometry
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